Romain Darnajoux

Romain Darnajoux

Post-doctoral Researcher in Biogeochemistry

Introduction

Romain Darnajoux
Princeton University, Geoscience Department romaind@princeton.edu Biogeochemistry Forest ecology Nitrogenase Cyanolichen Molybdenum Vanadium Boreal forest Nitrogen fixation Cyanobacteria

Education

  • Université de Sherbrooke
    2011 - 2015 PhD
    Biogeochemistry
    Sherbrooke University, Chemistry department Sherbrooke, QC, Canada Advisor : J.-P. Bellenger
    “Homeostasis of essential micronutrient for biological nitrogen fixation in the boreal trimembered cyanolichen Peltigera aphthosa”
  • Ecole Nationale Supérieure de Chimie de Lille
    2006 - 2010 MSc
    Chemistry
    National graduate School of Chemistry of Lille (ENSCL) Lille, France equivalent to a Master degree in Chemistry.
    · Courses in Spectroscopy, Chromatography, Organic chemistry, Analytical chemistry …
    · Erasmus year at the University of Kent, Canterbury, England (2009-2010) : Courses in Medicinal chemistry, Pharmacology, Human physiology and diseases.
  • Lycée François Ier
    2004 - 2006 Undergraduate
    Biology and Geology
    Lycée François Ier, CPGE Biology (French undergraduate selective program, equivalent to BSc) Fontainebleau, France French undergraduate selective program, equivalent to BSc : intensive courses in biology, chemistry, physics and earth’s sciences to prepare the competitive entrance examinations to French agronomy and chemistry leading schools “Grandes Ecoles”.

Download my Resume.

Research

Research interest

My research lies at the interaction between physical science, chemistry and biological sciences. I like to develop various physico-chemical methods and tools in order to decipher complex biological phenomenon. During my post graduate studies, I specialize myself in microbiology, especially cyanobacteria, and in the understanding of global biogeochemical pathway, such as nitrogen fixation. My goal is to further understand the abiotic and metabolic process that drive dynamics of N cycle, especially between cyanobacteria, and higher living form such as moss or lichen. I am also very interested in multivariate statistic and computer model for a better approach of complex emerging systems.

Research Experience

  • Princeton University
    Nov. 2016 - Present Simons Foundation Postdoctoral Fellow in Life Sciences Princeton University, Geosciences department Princeton, NJ, USA Co-advisors: F. Morel & F. Lutzoni (Duke University)
    “Deciphering the genomic and metabolic heterogeneity of biological nitrogen fixation in boreal ecosystems”
  • Université de Sherbrooke
    Sept.2015 - Sept.2016 Postdoctoral researcher Sherbrooke University, Biology department Sherbrooke, QC, Canada Advisor: R. Bradley.
    Postdoctoral researcher and coordinator of the Boreal Mini-FACE facility at Simoncouche
    “Studying the interaction of global changes with the N cycle in boreal ecosystems”
  • https://bellengerlab.wordpress.com/the-boreal-mini-face/

    Location of the Mini-FACE facility at Simoncouche

    The Boreal MiniFACE project is a joined effort between Universite de Sherbrooke (UdeS) and Universite du Quebec a Chicoutimi (UQAC) to better evaluate the impact of global climate change on nitrogen cycling in boreal feather mosses.
    The Boreal Mini Forced-Air CO2 Enriched Project at Simoncouche is located in Quebec, near the town of Saguenay.

    Technical scheme of the OTCs

    The MiniFACE facility includes 16 Open Top Chambers distributed in 4 subsites . Each subsite includes: a control OTC, an OTC under elevated CO2, an OTC with increased soil temperature and an OTC with both elevated CO2 and temperature. Each OTC is divided in 4 sections recieving 4 treatments; control, + phosphorus, + nitrogen and + micronutrients.

    - Close MiniFACE description -

  • Université de Sherbrooke
    2011 - 2015 PhD
    Biogeochemistry
    Sherbrooke University, Chemistry department Sherbrooke, QC, Canada Advisor : J.-P. Bellenger
    “Homeostasis of essential micronutrient for biological nitrogen fixation in the boreal trimembered cyanolichen Peltigera aphthosa”
  • Kent University
    April 2010 - Oct.2010 MSc project University of Kent, Biosciences Department Canterbury, England Advisor: G.K. Robinson, M.J. Howard
    “Toward the use of STD-NMR for ligand/microbial cell interaction.”

Awards

Scientific Communications

Publications

2018 :

7 - Darnajoux, R., Bradley, R., Houle, D., Bellenger, J. P. (2018) Predictable spatial patterns of biological nitrogen fixation in forest floor mosses: Color matters! Soil Biology and Biochemistry, 122, 160-62, doi: 10.1016/j.soilbio.2018.04.010

PDF

2017 :

6 - Darnajoux, R., Zhang, X., McRose, D. L., Miadlikowska, J., Lutzoni, F., Kraepiel, A. M. L. and Bellenger, J.-P. (2017), Biological nitrogen fixation by alternative nitrogenases in boreal cyanolichens: importance of molybdenum availability and implications for current biological nitrogen fixation estimates. New Phytologist, 213: 680–689. doi:10.1111/nph.14166

PDF

2016 :

5 - Zhang, X., McRose, Darnajoux, R., Bellenger, J. P., Morel, F.M.M., & Kraepiel, A.M.L. (2016) Alternative nitrogenase activity in the environment and nitrogen cycle implications. Biogeochemistry, 127(2), 189-98, doi:10.1007/s10533-016-0188-6.

PDF

4 - Jouogo-Noumsi, C., Pourhassan, N., Darnajoux, R., Deicke, M., Wichard, T., Burrus, V. & Bellenger, J. P. (2016) Effect of organic matter on nitrogenase metal cofactor homeostasis in Azotobacter vinelandii under diazotrophic condition. Environmental Microbiology Report, 8, 76–84 doi:10.1111/1758-2229.12353

PDF

2015 :

3 - Darnajoux R., Lutzoni F., Miadlikowska J., and Bellenger J. P. (2015). Determination of elemental baseline using peltigeralean lichens from Northeastern Canada (Québec): Initial data collection for long term monitoring of the impact of global climate change on boreal and subarctic area in Canada. Science of the Total Environment, 533, 1-7.

PDF

2014 :

2 - Darnajoux R., Constantin J., Miadlikowska J., Lutzoni F. and Bellenger J.P. (2014) Is vanadium a biometal for boreal cyanolichens? New Phytologist, vol202: 765-771

PDF

2013 :

1 - Allard P., Darnajoux R., Phalyvong K. and Bellenger J.P. (2013) Effects of tungsten and titanium oxide nanoparticles on the diazotrophic growth and metals acquisition by Azotobacter vinelandii under molybdenum limiting condition. Environmental Science and Technology, vol 47: 2061-2068

PDF

Invited speaker

  • Princeton University
    EGGS Lecture series (2014)
    Princeton University
    , NJ, USA
    “Deciphering the ecological importance of V-based nitrogen fixation in boreal ecosystems.”
  • Rutgers University
    ESGSA Special Seminar (2014)
    Rudgers University
    , NJ, USA
    “Deciphering the ecological importance of V-based nitrogen fixation in boreal ecosystems.”

Conferences

2018 :
Darnajoux, R., Renaudin, M., Magain, N., Lutzoni, F., Bellenger, J.-P., Zhang, X. “Vanadium nitrogenase in boreal cyanolichens: biome level activity and regulation.”. Oral presentation delivered at the 13th European Nitrogen Fixation Conference, Stockholm, Sweden (August 2018)
Darnajoux, R., Renaudin, M., Magain, N., Lutzoni, F., Bellenger, J.-P., Zhang, X. “Vanadium nitrogenase in boreal cyanolichens: biome level activity and regulation.”. Oral presentation delivered at the 17th International Society for Microbial Ecology Symposium, Leipzig, Germany (August 2018)
Darnajoux, R., Bradley, R., Le Monier, P., Houle, D., Bellenger, J.-P. “Drivers of spatial heterogeneity of biological nitrogen fixation in northeastern Canadian boreal forest.”. Poster presentation delivered at the Ecological Society of America 2018 meeting, New Orléans, LA, USA (August 2018)
2017 :
Darnajoux, R., Zhang, X., Magain, N., McRose, D., Miadlikowska, J., Kraepiel, A., Lutzoni, F., Bellenger, J.-P. “Vanadium nitrogenase in boreal cyanolichens: activity and regulation.”. Poster presentation delivered at the Goldschmidt 2017, Paris, France (August 2017)
Darnajoux, R., Zhang, X., McRose, D., Miadlikowska, J., Kraepiel, A., Lutzoni, F., Bellenger, J.-P. “A glimpse at the lichen symbiosis; from metal homeostasis to ecosystems function.”. Oral presentation delivered at the Northeastern Geobiology Symposium 2017, Storrs, Connecticut, USA (March 2017)
2016 :
Darnajoux, R., Zhang, X., McRose, D., Miadlikowska, J., Kraepiel, A., Lutzoni, F., Bellenger, J.-P. “The importance of vanadium-based nitrogen fixation in boreal cyanolichens: a case study using Peltigera aphthosa (L.) Willd. s. l.”. Poster presentation delivered at the 8th International Association of Lichenology Symposium, Helsinki, Finland (August 2016)
Darnajoux, R., Bradley Robert and Bellenger, J.-P. “In vivo characterization of nitrogenase kinetics in Anabaena variabilis ATCC 29413 using cavity ring-down spectroscopy”. Oral presentation delivered at the 12th Workshop on Cyanobacteria, Tempe, AZ, USA (May 2016)
Darnajoux, R., Houle, D., Bellenger, J.-P and Bradley Robert. “Fixation d’azote dans les pessières à mousse : Quand la couleur importe !”. Oral presentation delivered at the 10ème Colloque du Centre d’Étude de la Forêt, Montréal, Qc, Canada (May 2016)
2015 :
Jouogo Noumsi C., Darnajoux R., Pourhassan N., Deicke M., Wichard T., Burrus V. and Bellenger J.P. “Effect of natural organic matter on metal acquisition and nitrogenase use by Azotobacter vinelandii”. Oral presentation delivered at the International Symposium on Interactions of Soil Minerals with Organic Components and Microorganisms (ISMOM), Montréal, Qc, Canada. (July 2015)
Darnajoux R., Miadlikowska J., Lutzoni F. and Bellenger J.-P. “Le grand nord québécois demeure l’une des regions les moins contaminées en métaux de la planète”. Oral presentation delivered at the 19ème Colloque annuel du Chapitre Saint-Laurent, Sherbrooke, Qc, Canada. (June 2015)
Darnajoux R., Morin H., Lindo Z., Bradley R. and Bellenger J.-P. “The boreal mini-face project; l’effet des changement globaux sur la fixation biologique de l’azote en forêt boréale”. Poster presentation delivered at the 19ème Colloque annuel du Chapitre Saint-Laurent, Sherbrooke, Qc, Canada. (June 2015)
2014 :
Darnajoux R., Miadlikowska J., Lutzoni F. and Bellenger J.P. “Metal homeostasis in the foliose lichen Peltigera aphthosa from northern hemisphere.” Poster presentation delivered at the European Geology Union, General Assembly, Vienna, Austria. (July 2014)
Darnajoux R., Miadlikowska J., Lutzoni F. and Bellenger J.P. “Study of the importance of vanadium based nitrogen fixation in the tripartite boreal cyanolichen : Peltigera aphthosa.” Oral presentation delivered at the 18ème Colloque annuel du Chapitre Saint-Laurent, Quebec, QC, Canada.. (June 2014)
Jouogo Noumsi C., Darnajoux R., Pourhassan N., Deicke M., Wichard T., Burrus V. and Bellenger J.P. “Effect of natural organic matter on metal acquisition and nitrogenase use by Azotobacter vinelandii”. Poster presentation delivered at the American Society of Microbiology, General Meeting, Boston, MA, USA. (Mai 2014)
Darnajoux R. and Bellenger J.P. “Investigating temperature dependency of nitrogen fixation in cyanobacteria using ARACAS”. Poster presentation delivered at the Mer Bleue Annual Science Workshop, McGill University, Montréal, Qc, Canada. (March 2014)
2013 :
Bellenger J.P., Darnajoux R., Miadlikowska J., Lutzoni F. and Hodkinson B. “Metal homeostasis in the tri-membered lichen Peltigera aphthosa: another demonstration of the potential importance of vanadium to N2 fixation worldwide.” Oral presentation delivered at the 96th Canadian Chemistry Conference, Québec, Qc, Canada. (Mai 2013)
Darnajoux R., Bellenger J.P., Lutzoni F. and Miadlikowska J. “Determination of baseline metal contamination of foliose lichens in Eastern Canada (Québec).” Oral presentation delivered at the 96th Canadian Chemistry Conference, Québec, Qc, Canada. (Mai 2013)
2012 :
Allard P., Bellenger J.P., Darnajoux R., Phalyvong K. “Influence de nanoparticules metalliques sur la fixation d’azote par Azotobacter vinelandii.” Poster presentation delivered at the 16eme Colloque annuel du Chapitre Saint-Laurent, Quebec, QC, Canada (June 2012)

Outreach

Teaching